Translational_Unit
LuxOU

Part:BBa_K131016:Design

Designed by: Thane Kubik   Group: iGEM08_Calgary_Wetware   (2008-09-28)


LuxOU from Vibrio harveyi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 854
    Illegal SapI.rc site found at 816


Design Notes

LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The natural RBS was also kept.


Source

GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2

Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.

References